Akademik Veri Yönetim Sistemi
Cumhuriyet Medical Journal, 2022 (Hakemli Dergi)
Objective: Sugammadex (SUG) quickly reverses steroidal neuromuscular blocking drugs after anesthesia. It has
been reported that SUG has a toxic effect on neurons in primary culture. This study aims to examine the effect
of SUG on glial cell viability, oxidative stress, and apoptosis in C6 glial cells after exposure to H2O2.
Method: In this study, C6 glioma cell line was used to study the effect of SUG on the glial cell in four cell groups.
The control group was untreated. Cells in the H2O2group were treated with 0.5 mM H2O2 for 24 h. Cells in the
SUG group were treated with 50 μg/mL SUG for 24 h. Cells in the SUG+ H2O2 group were pre-treated with 50
μg/mL of SUG for 1 h before 24-h exposure to 0.5 mM H2O2. Cell viability was evaluated using XTT assay. Total
antioxidant status (TAS), total oxidant status (TOS), caspase-3, Bax, 8-hydroxy-2′ -deoxyguanosine (8-OHdG), and
cleaved-PARP levels in the cells were measured by commercial kits.
Results: SUG significantly decreased the viability of C6 cells after H2O2-induced oxidative stress (p < 0.05). SUG
pretreatment also raised TOS levels and led to increased Bax, Caspase-3, 8-OHdG, and cleaved PARP levels after
H2O2-induced oxidative damage in C6 cells (p < 0.05).
Conclusions: SUG is toxic agent on neurons and exacerbates H2O2-induced oxidative damage in C6 cells.