Equine arteritis virus is classified in the Arteriviridae family and causes reproductive and respiratory disorders. The host spectrum includes many species of the Equidae family. Horses, donkeys and mules are the most sensitive species. The infection was serologically investigated in adult donkeys on small private family type enterprises in eastern and south-eastern Anatolia in this study. A total of 1,532 samples were collected from 28 different locations in 6 different provinces in these two regions. The number of donkeys sampled from each farm was between 1 and 3. Serum samples were tested by indirect enzyme-linked immunosorbent assay (ELISA). As a result of sero-controls, 53 animals were positive (3.45%). The presence of infection was determined in all the provinces; Elazığ (7%, 17/241), Tunceli (2.4%, 3/122), Van (2.9%, 10/342), Bitlis (4.6%, 5/107), Şırnak (2.7%, 12/440) and Siirt (2.1%, 6/280). Seropositivity was detected in 22 of the 28 locations. In this study, data were obtained from a significant number of animals for the first time in these regions. Although the values were not high, the findings revealed the presence of infection in the majority of the investigated sites. Despite the fact that the incidence was not high in donkeys probably due to restricted management conditions, the incidence may increase over time and may pose a risk for thoroughbred horses unless necessary measures are taken.