Synthesis of crosslinked PVA/Chitosan hydrogels loaded EGF and evaluation in vitro characterization

Doğan M. , Sezer A. D.

Cumhuriyet Medical Journal, vol.42, no.1, pp.86-93, 2020 (International Refereed University Journal)

  • Publication Type: Article / Article
  • Volume: 42 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.7197/
  • Title of Journal : Cumhuriyet Medical Journal
  • Page Numbers: pp.86-93


Objective: The aim of this study was to prepare different hydrogels using chitosan and PVA polymers and to evaluate the cytotoxicity of hydrogels through cell culture studies. In addition, FTIR spectrum measurements were aimed to determine the specific chemical groups and bonds in the structures of hydrogels and materials.

Method: In this study, different hydrogels were prepared and 3- (4,5- dimethyl-2-thiazolyl) -2,5-diphenyl tetrazolium bromide (MTT) test was performed to evaluate the toxicity of hydrogels on mouse fibroblast cell line (L-929). In addition, specific chemical groups of materials and hydrogels used in hydrogels were determined via FTIR spectrum study.

Results: According to the MTT cytotoxicity results B1 (94.20±1.30 %) and D2 (82.45±1.05 %) formulations showed the greatest and lowest L-929 cell viability respectively. According to the results of the FTIR absorption spectrum, the aliphatic C = O tension band of rh-EGF was observed at 1634 cm-1 .

Conclusions: Results showed that chitosan-PVA hydrogels containing rEGF types were observed to increase mouse fibroblast cell viability. In the FTIR spectrum results, spectral bands showing specific chemical groups of chitosan, PVA, PEGylation agents, rh-EGF and C2 formulation were obtained.