Fatty acid profiles of total, neutral, and polar lipids in the liver, muscle, and intestine of female individuals of Vimba vimba (L., 1758) were determined by gas chromatography. C16:0, C16: 1 n-7, C18:1 n-7, and C18:1 n-9 were the most abundant fatty acids in total and neutral lipids in all tissues investigated. In addition to these acids, C20:4 n-6 (arachidonic acid) and C22:6 n-3 (docosahexaenoic acid) were the other fatty acids having high percentages in the polar lipid fraction of the tissues. The highest and lowest n-3 to n-6 ratios, which are a good indicator of the quality of fish oils, were determined in the muscle (1.68) and liver (0.68) in the polar lipid fraction, respectively. It was determined that the species had low levels of the n-3 forms of polyunsaturated fatty acids in the investigated tissues. For a better understanding of fatty acid metabolism of V. vimba, more detailed studies are required in terms of feeding experiments.