Investigation of the Effects of Alpha Adrenergic Receptors on Tumor Necrosis factor alpha and Interleukin-1Β levels in brain in pentylenetetrazole-induced kindled epileptic rats


Şahin B.

Gevher Nesibe 5.Uluslararası sağlık kongresi, Ankara, Türkiye, 24 - 25 Nisan 2020, cilt.1, no.1, ss.105

  • Cilt numarası: 1
  • Basıldığı Şehir: Ankara
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.105

Özet

Aim: Epilepsy is one of the major neurological diseases that affect about 1% of the world's population. The disease occurs due to periodic and spontaneous excessive electrical discharges of cortical and subcortical neurons. The alpha2-adrenoreceptor (AR) is the most investigated noradrenergic receptor with regard to modulation of seizure activity. However, the exact role of this receptor in modulating seizure activity is not clear.. The aim of this study is to investigate tumor necrosis factor alpha (TNF-α) and interleukin-1 (IL-1β) levels in the brain of rats in the pentylenetetrazole (PTZ) induced epileptic models in rats. Material and Method: In our study, 24 male 220-240 grams Wistar Albino rats were used. Rats was divided into four groups as control (n=6), Pentylenetetrazole (n=6, PTZ 35 mg / kg PTZ was injected every other day to each group intraperitoneally (i.p). According to Racine scale, animals with 3 seizures at stage 5 and above were considered to be kindled) alpha-2 agonist (dexmedetomidine, 1 mg/kg, i.p, n=6), alpha-2 antagonist (atipamezol, 0.75mg/kg, i.p, n=6). The brain tissues of the animals were removed. After the tissues were homogenized, TNF-α and IL-1β levels were measured in these tissues by using ELISA (enzyme-linked immunosorbent assay) method. The statistical evaluation was done with one-way ANOVA and multiple comparisons were determined by the Tukey test. Results: There was significance increase in TNF-α levels in PTZ group compared to control (p<0.05). There was a significant decrease in TNF-α in Alfa-2 antagonist group compared to PTZ group (p<0.05). Moreover, there was significance increase in IL-1β levels in PTZ group compared to control (p<0.05). However, Alfa-2 agonist reduced IL-1β compared to PTZ (p<0.05). Conclusion: It was found that TNF- α was decreased by the Alfa-2 antagonist and IL-1β is reduced by the Alfa-2 agonist in PTZ kindled epileptic model in rat brain. Keywords: Epilepsy, Pentylenetetrazole, Alfa-2 Adrenergic Receptor, Tumor Necrosis Factor Alpha, Interleukin-1β, Rats