Akademik Veri Yönetim Sistemi
INTERNATIONAL MULTIDISCIPLINARY SCIENTIFIC RESEARCH CONGRESS, Sivas, Türkiye, 4 - 06 Temmuz 2025, cilt.2, ss.667-677, (Tam Metin Bildiri)
colon cancer is one of the leading causes of cancer-related deaths. Since chemotherapeutic agents used
in cancer treatment can cause serious side effects, the search for natural and non-toxic alternatives is
gaining increasing importance. Chondrus crispus (Irish moss, carrageenan) is a red seaweed native to
the North Atlantic coasts and important due to its rich bioactive components. The aim of this study was
to evaluate the anticancer and antioxidant effects of dried ethanol extract of C. crispus, alone and in
combination with docetaxel, on colon cancer HT-29 cell line under in vitro conditions. Anticancer
activity was determined by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. HT-29
cells were incubated with concentrations ranging from 1–75 μg/mL for 24, 48 and 72 hours to evaluate
the anticancer activity of C. crispus. With the same method, C. crispus ethanol extract was combined
with docetaxel to investigate anticancer activity in cells where it was aimed to create a synergistic effect.
To evaluate antioxidant activity, total phenolic content was determined by Folin-Ciocalteu method, free
radical scavenging effect was determined by 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) method, and
iron reducing capacities were determined by Ferric Reducing Antioxidant Power (FRAP) analysis.
According to the findings, the anticancer activity of the dried form of C. crispus plant ethanol extract
and docetaxel combination was found to be high due to the decrease in cell viability compared to cells
administered only C. crispus or docetaxel. In addition, it was determined by DPPH and Folin-Ciocalteu
analysis that the combination of C. crispus with docetaxel showed the highest antioxidant activity. In
FRAP analysis, it was determined that the iron (Fe³⁺) reducing capacity of the extract was only in cells
administered docetaxel. As a result, it was determined that the combination of C. crispus ethanol extract
with docetaxel increased the anticancer and antioxidant activity in the HT-29 cell line.
Keywords: Anticancer activity, antioxidant activity, Chondrus crispus, HT-29 cell line, colon cancer