Isolation and identification of chitin from heavy mineralized skeleton of Suberea clavata (Verongida: Demospongiae: Porifera) marine demosponge


Ehrlich H., Bazhenov V. V. , Debitus C., de Voogd N., Galli R., Tsurkan M. V. , ...Daha Fazla

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.104, ss.1706-1712, 2017 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 104
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1016/j.ijbiomac.2017.01.141
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Sayfa Sayıları: ss.1706-1712

Özet

Since the discovery of chitin in skeletal structures of sponges (Porifera) in 2007, studies on search of novel species which possess this structural aminopolysaccharide continue up today. The most potential source of chitin is suggested to be localized in the four families of sponges related to the order Verongida (Demospongiae) which nevertheless require further clarification. Here, we report for the first time the isolation and identification of alpha-chitin from the Suberea clavata demosponge (Aplysinidae: Verongida). Raman spectroscopy, Calcofluor White staining, chitinase test and ESI-MS techniques were used to identify chitin. We suggest that the presence of chitin within fibrous skeletons of diverse species of Verongida order, and, especially in all species of the Aplysinidae family, may be useful for the identification of novel, previously unidentified marine demosponges. (C) 2017 Elsevier B.V. All rights reserved.