Akademik Veri Yönetim Sistemi
International Journal of Scientific and Technological Research , cilt.7, sa.8, ss.77-84, 2021 (Hakemli Dergi)
Purpose: The current study was intended to investigate the potential effect of ethosuximide on
glutamate-induced cytotoxicity and oxidative stress in C6 glial cells.
Material and Methods: In our study, the C6 cell line was used. To see how ethosuximide affected
glutamate-induced cytotoxicity, four cell groups were created. The control group was without any
treatment. Cells in the glutamate group were exposed to 10 mM glutamate for 24 h. Cells in the
ethosuximide group were treated with various concentrations (5, 10, 20, and 40 μM) of ethosuximide
for 24 h. Cells in the ethosuximide + glutamate group were pre-treated with various concentrations of
ethosuximide for 1 h and then treated with glutamate for 24 h. The cell viability was measured using an
XTT assay.Total oxidant status (TOS) and Total antioxidant status (TAS) in the cells were measured by
commercial kits.
Results: Ethosuximide at the concentrations of 10, 20, and 40 μM significantly increased the cell
viability in C6 cells (p < 0.001). Ethosuximide at the concentration of 40 μM significantly decreased
the TOS levels (p < 0.05) whereas didn’t alter the TAS levels (p > 0.05).
Conclusion: Ethosuximide can cause neuroprotective effect on C6 cells. Additionally, one of the
potential mechanisms involved in this effect is inhibition of oxidative stress.
Keywords: Ethosuximide, Oxidative Stress, cell viability, C6 Glioma.