Adverse effects of oxytetracycline and enrofloxacin on the fertility of Saanen bucks

Yucel U. M., Koşal V., Kara M., Taspinar F., Uslu B. A.

TROPICAL ANIMAL HEALTH AND PRODUCTION, cilt.53, sa.5, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53 Sayı: 5
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s11250-021-02898-7
  • Dergi Adı: TROPICAL ANIMAL HEALTH AND PRODUCTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Oxytetracycline, Enrofloxacin, Saanen buck, Sperm DNA damage, Spermatological parameters, Testosterone, TROUT ONCORHYNCHUS-MYKISS, SPERM QUALITY, ANTIBIOTICS, TETRACYCLINE, PARAMETERS, TISSUE, AMINOGLYCOSIDES, MARBOFLOXACIN, ERYTHROMYCIN, OFLOXACIN
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

This study aimed to determine the adverse effects of oxytetracycline and enrofloxacin application on the fertility of Saanen bucks. For this purpose, twenty-four bucks were divided into three groups. Group I (control group) received only 5 ml of 0.9% NaCl for 7 days, group II was given a single dose of 20 mg/kg oxytetracycline and group III was given at a dose of 2.5 mg/kg per day for 7 days intramuscularly. Serum and semen samples were collected from the bucks at post-treatment 1, 3, 5, 7, and 9 days and examined spermatological parameters (quantity, motility, density, abnormal sperm ratio, and live-dead sperm ratio), serum testosterone levels (with ELISA) and sperm DNA parameters (with Comet assay). The results showed no change in sperm volume, abnormal sperm rate, and dead-live sperm ratio in group II and III following oxytetracycline and enrofloxacin administration. However, a decrease in sperm density, sperm motility, mass activity, and testosterone levels, and an increase in sperm DNA damage were detected. These spermatological parameters (density, motility, mass activity) and testosterone levels were less decreased and sperm DNA damage was less increased in group II than group III. The greater damage in group III may be attributed to the longer duration of enrofloxacin administration compared to oxytetracycline and the effect of enrofloxacin on DNA. The results obtained from this study suggest that usage of oxytetracycline and especially enrofloxacin should be restricted and antibiotics with fewer side effects on sperm should be preferred in Saanen bucks during the reproduction period.