The study was conducted to determine the effects of different rootstocks (Ma x Ma 14, GiselA 5, GiselA 6 and SL 64) on quality properties and bioactive compounds of '0900 Ziraat' sweet cherry fruit. Fruit weight, thickness and length of Ma x Ma 14 and SL 64 was higher than the GiselA 5 and GiselA 6. Firmness of GiselA 6 was lower than the other rootstocks (Ma x Ma 14, GiselA 5 and SL 64). The chroma value of GiselA 5 was greater than the other rootstocks, whereas the hue angle of SL 64 was lower than the other rootstocks. While the lowest SSC and acidity was obtained in GiselA 5, the highest vitamin C was determined in Ma x Ma 14. Total phenolics and total flavonoids, thus antioxidant activity of GiselA 6 and SL 64 rootstocks was higher than Ma x Ma 14 and GiselA 5 rootstocks. Catechin was the major phenolic acid in '0900 Ziraat' sweet cherry fruit. GiselA 6 had higher catechin and 4-hydroxybenzoic acid content than the other rootstocks. As a result, it was revealed that sweet cherry rootstocks were effective on bioactive compounds and fruit quality characteristics.