In this study, the effects of Sunset Yellow, Ponceau 4R, Allura Red, Brilliant Blue FCF, and Brown HT on the longevity of Drosophila melanogaster was investigated. The effects of different concentrations of these food dyes (0.5 mg(-1) mL, 1 mg(-1) mL, 1.5 mg(-1) mL, and 2 mg(-1) mL) were separately administered one by one to female and male populations of Drosophila melanogaster for application groups. In all of application groups of each population the longevity decreased, depending on the concentrations of food dyes. It was found that the difference between the groups was significantly important (p<0.05). We observed that Brilliant Blue FCF caused the biggest decreased in life span amoung the other food colouring dyes followed by Ponceau 4R, Sunset yellow, Brown HT and Allura Red during this study.