Akademik Veri Yönetim Sistemi
2. INTERNATIONAL CANCER DAYS (2ND ICD), Sivas, Türkiye, 16 - 18 Eylül 2021, ss.326-327
It is seen that bee products are frequently used with their therapeutic qualities in folk medicine as a
natural medicine in many parts of the world [1]. It has been demonstrated that natural bee products like honey,
pollen, propolis etc. and their isolates have wide spectrum of biological activities such as antimycotic,
antioxidant, antitumor, anti-inflammatory, cytotoxic, anti-ulcer and immunomodulatory activities and
cardioprotective properties [2-5]. Today, the use of bee products in medical applications, their use as a food
supplement in food and beverage substances, and their use as a source of antioxidant and antiaging in the
cosmetic industries are becoming extremely common. Studies in the literature have shown that there are
significant differences between the contents and biological activities of bee pollen from different botanical
origins and different regions [6-7]. Sivas province is a city with high potential in terms of beekeeping. At the
same time, Sivas province has a very convenient structure for beekeeping activities in terms of natural plant
diversity and land structure. However, scientific studies on bee products of Sivas province are very limited. In
this study, it was aimed to determine and compare the antiproliferative activity of bee pollen samples collected
from central, Yıldızeli and Zara districts of Sivas province in SK-OV-3 (Human ovarian cancer) cell line. Pollen
samples were obtained from beekeepers in the 2020 harvest period, and extractions were carried out in the
laboratory using ethyl alcohol and water solvents. Antiproliferative activities of isolates were investigated in
SK-OV-3 cell line using XTT cell proliferation kit. XTT results were determined spectrophotometrically and
IC50 values were calculated with the help of numerical data obtained by Graphpad analysis program. It was
observed that both ethyl alcohol and water isolates of pollen samples showed good antiproliferative activity in
SK-OV-3 cell line (IC50 < 100 μg/mL). It is the first time to test the efficacy of pollen samples on the SKOV-
3 cell line. It is thought that this study will be a beginning in terms of the therapeutic use of bee pollen samples
from Sivas province and the preparation of new effective supplements.
Keywords: Antiproliferative activity, bee products, SK-OV-3, pollen, Sivas.