The present paper describes a green and cost-effective approach to investigate chitosan-sepiolite (Ch-Sep) composite as an adsorbent for removal of UO22+ ions in aqueous solution. The Ch-Sep composite was prepared as a beads using with two cross-linking agents: tripolyphosphate (TPP) and epichlorohydrin (ECH). Their adsorption properties for the removal of UO(2)(2+ )ions in aqueous solution by batch experimental conditions were studied. The adsorptive removal processes of UO(2)(2+ )ions from aqueous solution were evaluated by Langmuir, Freundlich and Dubinin-Radushkevich isotherm models, and was found to be perfectly fit to the Langmuir model (R-2 = 0.971). The maximum adsorption capacity was 0220 mol kg(-1) at 25 degrees C from Langmuir isotherm model. Adsorption energy was 12.1 kJ mol(-1) indicating that the adsorption process was chemical. The adsorption kinetics followed the pseudo second order and infra particle diffusion models. The thermodynamics parameters of UO22+ ions removal from aqueous solution was confirmed spontaneous, endothermic and possible at higher temperatures behavior of adsorption process. The adsorption mechanism of UO(2)(2+ )ions onto Ch-Sep composite beads was investigated by FT-IR and SEM analysis. These findings revealed the effectiveness and potential of the newly synthesized Ch-Sep composite beads for the removal of UO22+ ions. (C) 2021 Elsevier B.V. All rights reserved.