Detection of Chlamydophila abortus in ovine milk by Immunomagnetic separation-polymerase chain reaction

Ongor, H; Cetinkaya, B; Acik, MN; Karahan, MURAT; Bulut, H

doi:10.1046/j.1439-0450.2003.00716.x

Detection of Chlamydophila abortus in ovine milk by Immunomagnetic separation-polymerase chain reaction

Ongor H., Cetinkaya B., Acik M., Karahan M., Bulut H.

JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, cilt.51, sa.1, ss.43-45, 2004 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 51 Sayı: 1
Basım Tarihi: 2004
Doi Numarası: 10.1046/j.1439-0450.2003.00716.x
Dergi Adı: JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.43-45
Sivas Cumhuriyet Üniversitesi Adresli: Hayır

Özet

The present study was carried out to investigate the presence of Chlamydophila abortus, the causative agent of ovine enzootic abortion, in milk samples collected from sheep flocks with and without the history of abortion in Eastern Turkey by means of immunomagnetic separation (IMS) in conjunction with the polymerase chain reaction (PCR). A total number of 201 milk samples collected from 10 flocks with abortion and four flocks without abortion were tested. In the analysis of the milk samples by IMS-PCR, correct amplification was obtained with three (1.5%) samples originating from one flock with abortion. In the digestion of PCR positive products by AluI, restriction profiles observed in all three samples were determined to be the same as C. abortus S26/3.