Molecular characterization of the bovine noroviruses from diarrheic calves in Turkey


Gulacti I., SÖZDUTMAZ I., IŞIDAN H.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.40, ss.428-433, 2016 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 40 Konu: 4
  • Basım Tarihi: 2016
  • Doi Numarası: 10.3906/vet-1504-67
  • Dergi Adı: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Sayfa Sayıları: ss.428-433

Özet

In this study, we performed a survey to detect bovine noroviruses (BNoVs) in Turkey between 2009 and 2011 using 235 fecal samples from neonatal calves with diarrhea that were analyzed by nested reverse-transcription RT-PCR, using primers located in the consensus sequences of the BNoV RNA-dependent RNA polymerase (RdRp) gene. The BNoV RdRp gene was detected in 1.7% (4/235) of samples by nested RT-PCR. The nucleotide sequences of partial RdRp fragments from the BNoV isolates including the newly identified Turkish isolates showed more than 86.3% nt and 96.8% aa identity with each other. Interestingly, in a comparison of the nt and aa sequences, one of the Turkish BNoV strains was found to be 100% aa identical with some Italian and Tunisian strains. In conclusion, BNoV is one of the pathogens that contributes to neonatal calf diarrhea cases in Turkey.