In this study, the presence of infectious pancreatic necrosis virus (IPNV) was investigated in farmed rainbow trout in different regions of Turkey. For this purpose, total of 243 isolation materials (47 seminal fluids, 62 ovarian fluids and 134 fingerling) were collected from 30 farms in the period of December and March 2010-2011. Isolation materials were passaged twice in BF-2 (Bluegill fry-2) cell cultures for virus isolation. The cell culture supernatants showed cytopathic effect (CPE) were tested by antigen-capture enzyme linked imnnunosorbent assay (Ag-ELISA) for IPNV identification. All isolation materials were tested also by reverse transcriptase polymerase cahin reaction (RT-PCR) in order to compare with the test of the virus isolation. IPNV was detected from 26(10.7%) of total 243 isolation materials obtained from 17 (56.6%) of 30 farms in 7 (63.6%) of 11 provinces. In addition, that RT-PCR test can be used as an alternative method in the IPNV-diagnosis by directly tissue-testing due to less expensive and faster than the virus isolation method was determined.