This study was conducted to determine the effects of hesperidin, a flavonoid added to quail diets, on blood serum, enzymes in tissues, intestinal histomorphology and fecal microflora. In the study, first treatment [(control) (0g hesperidin/kg feed)], second treatment [(HES1) (1 g hesperidin/kg feed)], third treatment [(HES2) (2 g hesperidin/kg feed)] was added to with the basal diet through 35 d. The study was carried out with 3 main groups, 20 quails with 5 sub-repeats in each group and a total of 300 quails. At the end of the study, blood, liver and thigh muscle tissue and fecal samples were taken. Alanine transaminase (ALT) and lactate dehydrogenase (LDH) decreased in the HES1 group but increased in the HES2 group compared to the control group (P<0.05). Aspartate transaminase (AST) increased in the HES1 group compared to the control group and decreased in the HES2 group (P<0.05). Amylase, on the other hand, showed a regular increase in HES1 and HES2 groups to which hesperidin was added to the control group in hesperidin added groups (P<0.05). The tissue antioxidant GSH, CAT and SOD enzyme parameters showed a significant increase in the hesperidin added groups compared to the control group, and this increase was found to be significant compared to the control group (P<0.05). In intestinal histomorphology, in hesperidin treatment groups increased the height of villus in the cecum tissue; in colon tissue, it was determined that hesperidin added groups increased villus height but decreased crypt depth (P<0.05). Consequently, diets hesperidin with treatments positively is thought to affect the lipid, thigh, liver and serum antioxidant enzyme levels, intestinal histomorphology and fecal microflora in quail.