Given the pharmacological significance of 8-quinolinols and benzimidazoles, in the present paper, two series of new N-Heterocyclic having 8-quinolinol and benzimidazole moieties within a single molecular framework were prepared and characterized by elemental analysis, IR, and 13C/1H NMR techniques. To evaluate the desired compound as DNA-binder 3a, 3d, 7a, and 7d were docked with 1BNA DNA using AutoDock version 4.2. On the other hand, many proteins that are crystal structure of the BRCT repeat region from the breast cancer-associated protein, BRCA1 (ID: 1JNX), structure of a b-DNA dodecamer (ID: 1BNA), crystal structure of VEGFR kinase (liver cancer) protein (ID: 3WZE), and crystal structure of an allosteric Eya2 phosphatase inhibitor (lung cancer) protein versus (ID: 5ZMA) proteins, were used to compare the biological activities of all molecules using Maestro Molecular modeling platform. Afterward, ADME/T analysis of the molecules was performed. The derivatives of two series and Nitroxoline drugs were assessed for in vitro antibacterial activity against four microorganisms, including, two gram (+)bacteria such as B. subtilis, S. aureus, and two gram (-)bacteria such as E. ludwigii, E. coli. All derivatives were found to have moderate to good antibacterial potential. Of the 9 derivatives, 7d has significant antibacterial potential with MIC values of below 20 mu g/mL comparable to Nitroxoline vs. all bacteria.