TAGT-v1: a proposed protocol for transgenerational cytogenotoxicity assessment in plant bioassays
Environmental Science and Pollution Research, 2026 (Scopus)
- Yayın Türü: Makale / Derleme
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s11356-026-38061-8
- Dergi Adı: Environmental Science and Pollution Research
- Derginin Tarandığı İndeksler: Scopus, IBZ Online, ABI/INFORM, BIOSIS, Compendex, EMBASE, Environment Index, Geobase, MEDLINE, Natural Science Collection (ProQuest), Biomedical Reference Collection: Corporate Edition (EBSCO), Health Research Premium Collection (ProQuest), Pharma Collection (ProQuest)
- Anahtar Kelimeler: Cytogenetic endpoints, Ecotoxicology, Epigenetic inheritance, Multigenerational assessment, Pesticide risk assessment, Plant bioassay, Transgenerational genotoxicity
- Sivas Cumhuriyet Üniversitesi Adresli: Evet
Özet
While plant bioassays are widely used for acute genotoxicity, transgenerational effects are predominantly studied in animal models. A comprehensive literature review revealed a critical gap in plant-based transgenerational cytogenotoxicity studies. To bridge this gap, I propose the TAGT-v1 (Transgenerational Assessment of Genotoxicity in Plants, version 1) protocol as the first proposed standardized transgenerational plant bioassay framework. TAGT-v1 evaluates intergenerational genotoxic effects via seed-to-seed transfer (F0 → F1 → F2 → F3) following a single-point exposure in the F0 generation. The protocol employs short-life-cycle species (Hordeum vulgare, Rapid-cycling Brassica rapa, Brachypodium distachyon, and Arabidopsis thaliana) and excludes clonally reproducing models. Offering an ethical, cost-effective, and ecologically relevant alternative to animal testing, TAGT-v1 assesses both acute F0 effects and heritable genomic damage in the F3 generation—operationally defined as the first fully independent generation after F0 seed exposure, provided that chemical carry-over is excluded by LC–MS/MS. By overcoming the limitations of single-generation approaches, TAGT-v1 enables a more realistic evaluation of the long-term, population-level impacts of environmental pollutants. Future research will focus on the experimental validation of this protocol.