Selective and single step adsorption of a-lactalbumin from whole cow's milk on hydroxyapatite microbeads

ÇETİNKAYA S., AKKAYA B.

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, vol.68, pp.573-578, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 68
  • Publication Date: 2016
  • Doi Number: 10.1016/j.msec.2016.06.040
  • Journal Name: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.573-578
  • Keywords: Alpha-lactalbumin, Hydroxyapatite, Milk proteins, Selective adsorption, Polymerization, PROTEIN ALPHA-LACTALBUMIN, MIXED MATRIX MEMBRANES, NEUTRAL AMINO-ACIDS, WHEY PROTEINS, BETA-LACTOGLOBULIN, EXCHANGE CHROMATOGRAPHY, LIQUID-CHROMATOGRAPHY, VULNERABLE SUBJECTS, ANION-EXCHANGE, PURIFICATION
  • Sivas Cumhuriyet University Affiliated: Yes

Abstract

In this study, newly modified hydroxyapatite microbeads were used to isolate alpha-lactalbumin (alpha-LA) from cow's milk by a single step. Inorganic material, pure hydroxyapatite was embedded in ethylene glycol dimethacrylate (EGDMA) in the presence of pore maker. Newly synthesized hydroxyapatite microbeads were used firstly for adsorption of alpha-LA from aqueous solution. The maximum adsorption capacity of alpha-LA from aqueous solution was observed at pH 7.0 (10.0 mM phosphate buffer) at 25 degrees C in a batch system, in which parallel separate experiments were performed independently. Synthesized hydroxyapatite microbeads and pure hydroxyapatite were used for adsorption studies from whole milk (without pre-treatment such as fat and caseins removal). Our method is cheap, simple, efficient and rapid. The results showed that modified hydroxyapatite microbeads had selective affinity to alpha-LA because of good adsorption properties. (C) 2016 Elsevier B.V. All rights reserved.