Akademik Veri Yönetim Sistemi
Journal of Drug Delivery Science and Technology, cilt.122, 2026 (SCI-Expanded, Scopus)
The periosteum is a specialized connective tissue that plays a critical role in bone formation and repair due to its complex architecture, diverse cellular composition, and osteogenic potential. However, the therapeutic use of autologous or allogeneic periosteum is limited by donor site morbidity, immunological rejection, and infection risk. Tissue-engineered periosteum constructs have faced challenges, including low cellular proliferation, high scaffold rigidity, limited bioactivity, and incomplete replication of native periosteal structure and function. To address these issues, this study reports the fabrication of an economical trilayer biomimetic tissue-engineered periosteum (TEP) using a combination of electrospinning and freeze-drying. The TEP comprises a chitosan (CH)/hydroxyapatite (HAp) layer to enhance osteoconductivity and cell adhesion, a polyurethane (PU) layer to provide mechanical strength and flexibility, and a biodegradable PU layer functionalized with rosemary oil (RO) to confer antimicrobial properties and potential relevance to vascularization. In vitro characterization demonstrated that the TEP exhibits suitable mechanical properties, an interconnected porous architecture, and good cytocompatibility, supporting MC3T3 osteoblast adhesion and proliferation. These results highlight the scaffold's potential to serve as a supportive microenvironment for periosteum-inspired tissue engineering and provide a basis for future studies using more advanced biological models.