Aim: The purpose is to evaluate the relationship between Restless legs syndrome (RLS) and 25-Hydroxy D [25 (OH) D] vitamin level among hemodialysis patients. Material and Method: Hemodialysis patients, in Cumhuriyet University Faculty of Medicine Department of Nephrology were included in this study. Patients were asked about the diagnosis criteria of the International RLS study group and their laboratory examinations along with 25 (OH) D vitamin levels were assessed. Results: Seventy-five patients, 40 of whom were female, were included in the study. The average age of patients was 57.8 (19-84). Average 25 (OH) vitamin D levels of 75 patients in total were found to be 12.6 +/- 6.27 (3-30). Five patients had 25 (OH) D vitamin insufficiency and 70 had 25 (OH) D vitamin deficiency. Thirty-three patients were diagnosed with RLS and their average vitamin D levels were 10.76 +/- 4.56; the D levels of 42 patients not diagnosed with RLS were found to be 14.18 +/- 7.02. A significant relationship was determined between the patients diagnosed with RLS and their vitamin D levels (p=0.018). Discussion: A significant relationship was determined between RLS and 25 (OH) vitamin D level among hemodialysis patients. The fact that frequency of RLS was higher among hemodialysis patients compared to the general population may be related to low levels of 25 (OH) D vitamin.