Karyotype analysis was performed on Alburnus heckeli (Battalgil, 1943) (Earn: Cyprinidae) by investigating the number and structures of its chromosomes. The fish used in this study were caught with fishing nets in Lake Hazer and were taken to our laboratory. Fish were injected intraperitoneally with doses of 0.01 ml/g body weight of 0.6% colchicine solution and left for 190 min before sacrificing. It was determined by metaphase investigation that A. heckeli has 2n = 50 chromosomes. Its karyotypes were determined to be composed of 7 metacentric, 9 submetacentric and 9 acrocentric chromosome pairs with NF = 82. We were unable to identify any sex-related chromosomes in this species.