In the field of anatomy, preservation techniques used to preserve the integrity of specimens prepared for use as educational and research material are very ancient. It is desirable that these materials be as close as possible to the characteristics of living animal tissues in order to be an effective educational material. One of the most important criteria in determining similarity to fresh cadavers is the color and odor changes in the tissues. Therefore, the aim of this study is to reveal the anatomical and physiological changes on myocardial tissue, fixed by 4 different solutions in which qualitative and quantitative methods such as color analysis, sensory analysis, and microbiological tests. As a result, it was determined that a newly developed enriched saline solution and low concentration formaldehyde solution can be used safely for the preparation, preserve and long term use of educational materials.