In this study, tetracycline adsorption using the biocomposites prepared by the immobilization of chitosan polymer and Candida yeasts (Candida lipolytica, Candida membranaefaciens, Candida tropicalis, Candida utilis) and Rhizopus delemar fungus as a microorganism on chitosan was examined. In the used parameter ranges, temperature was determined as 25-550 degrees C, pH as 2-7, concentration as 10-100 mg / L, optimum pH value as 4.0 and optimum temperature as 25 degrees C, maximum capacity chitosan-Candida utilis biocomposite 1.179 mg / g as determined. The experimental equilibrium data were adjusted to the Freundlich isotherm model of chitosan and biocomposites and the kinetic data were adjusted to the pseudo-first-order kinetics model. Enthalpy, entropy and Gibbs free energy change of adsorption processes operating according to the thermodynamic parameters were determined to be exothermic and spontaneous. Chitosan-Candida utilis biocomposite for before and after adsorption FTIR, SEM analysis and characterization studies was realized. This study proved that the new PAN-Candida utilis biocomposite might emerge as alternatives for the removal of tetracycline.