Akademik Veri Yönetim Sistemi
Pharmacological Research - Natural Products, cilt.9, 2025 (Scopus)
The chemical compounds of aerial parts from three acclimated oreganos, derived from in vitro culture of Origanum compactum Benth. and Origanum vulgare L. clones 1 and 2, were investigated. First, we identified the best extraction solvent, followed by the determination of total phenolic and flavonoid contents. Subsequently, the total antioxidant status, total oxidant status, and oxidative stress index of the three species were assessed. Finally, the separation and identification of the different chemical compounds of the hydro-methanolic extracts were carried out by gas chromatography coupled with mass spectrometry. The results showed that methanol 80 % is the most suitable for the extraction of phenolic compounds (62.12 SAE/g dry matter). Dry powders from the aerial parts of the three acclimatized species contain up to 54.88 mg gallic acid equivalent/g dry matter for polyphenols and 9.2 mg quercetin equivalent/g dry matter for flavonoids. Analysis of the antioxidant activity revealed that the total antioxidant status of the three extracts is between 2.98 and 3.63 mmol Trolox equivalent/L, the highest value being observed in O. vulgare clone 2. The total oxidant status is between 22.95 and 44.45 µmol H2O2 equivalent/L, the highest value being found in O. vulgare clone 1. The oxidative stress index is between 1.01 and 1.21 AU, the highest value being observed in O. vulgare clone 2. The GC-MS of the hydro-methanolic extracts demonstrated the presence of 52 peaks and made it possible to determine three major categories of compounds: polyphenols, essential oil, and fatty acids. The two oreganos have the same volatile compounds with different chromatographic profiles.