Seasonal variations in the liver and muscle fatty acid composition of Alburnus chalcoides (Guldenstadt, 1772) from Todurge Lake (Sivas, Turkey) were determined. The most abundant fatty acids in the investigated seasons and tissues were C16:0, C18:0, C18:1 n-9, C18:1 n-7, C20:4 n-6, C20:5 n-3, and C22:6 n-3. The total saturated fatty acids (SFAs) of the liver and muscle were at the highest in the spring (38.59%) and summer (41.65%), respectively. The total monounsaturated fatty acids (MUFAs) were at the highest in the autumn (51.79%) for liver and in the winter (40.04%) for muscle. The total polyunsaturated fatty acids (PUFAs) were determined to be at the lowest levels in the autumn for the both liver and muscle. The lowest total n-6 PUFA levels were determined as 2.60% and 4.19% in the autumn for the liver and muscle, respectively, while the lowest total n-3 PUFA levels were 14.84% for the liver (autumn) and 22.60% for muscle (spring).