Preparation of sulfatide mimicking oleic acid sulfated chitosan as a potential inhibitor for metastasis


Kocabay S., AKKAYA B.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, cilt.147, ss.792-798, 2020 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 147
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.ijbiomac.2019.10.119
  • Dergi Adı: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Sayfa Sayıları: ss.792-798

Özet

Sulfatide is associated with numerous health problems, affecting different parts of the human body, including the metastasis; however, the underlying mechanisms are yet to be fully elucidated. Sulfatide has been used to potential inhibitor for tumor cell metastasis. In the present study we synthesized oleic acid sulfated chitosan (OlcShCs). It shows structural similarity to sulfatide because of its functional groups (sulfate and fatty acyl chains). Chitosan has smart properties such as biocompatibility, biodegradability and non-toxicity. We have prepared oleic acid sulfated chitosan (OlcShCs) by chitosan modification to mimic sulfatide. Its structure was characterized by FT-IR, H-NMR, and thermogravimetric analysis. After characterization studies its antimicrobial, antifungal and cytotoxic properties were investigated. Oleic acid sulfated chitosan (OlcShCs) was tested for its anti-cancer potential against human cancer cell lines (HeLa (ATCC (R) CCL-2 (TM))) for 24 h, 48 h and 72 h using the MIT assays. This new material which is soluble at physiological conditions, is a potential candidate for further metastasis inhibition investigations. (C) 2019 Elsevier B.V. All rights reserved.