Mitochondria were deposited via dip coating on different substrates, namely, commercial glass, copper plate (Cu), aluminum plate (Al), and poly (methylmethacrylate). Mitochondria are organelles that are found in live cells. Mitochondria produce most of the ATP via oxidative phosphorylation. First, mitochondria were extracted for the thin film, as described in the literature. Capacitance properties of thin films were measured with Agilent LCR meter. SEM analysis was used for the surface analysis. The structure of mitochondrion was characterized with FTIR, whose spectrum was measured using a Perkin Elmer Spectrum 400 spectrometer. The stretching vibration of the P-O bond was observed at 1236 cm(-1) in FTIR spectrum of the thin film. The capacitance and dielectric permittivity were first measured and calculated for the substrates, and then for mitochondria plated substrates. The properties, such as dielectric permittivity, capacitance, energy density, and power density of some substrates have been changed when their surface has been coated with mitochondria. Published by AIP Publishing.