Atıf İçin Kopyala
Değerli S., Değerli N., Çeliksöz A., Özçelik S.
TURKISH JOURNAL OF MEDICAL SCIENCES, cilt.42, ss.1268-1272, 2012 (SCI-Expanded)
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Yayın Türü:
Makale / Tam Makale
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Cilt numarası:
42
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Basım Tarihi:
2012
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Doi Numarası:
10.3906/sag-1104-32
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Dergi Adı:
TURKISH JOURNAL OF MEDICAL SCIENCES
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Derginin Tarandığı İndeksler:
Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
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Sayfa Sayıları:
ss.1268-1272
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Anahtar Kelimeler:
Giardia intestinalis, gdh, tpi, RFLP, genotyping, subgroups, DUODENALIS, HUMANS, PCR, CRYPTOSPORIDIUM, TRANSMISSION, EXCYSTATION, ASSEMBLAGES, BIOLOGY, GENE
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Sivas Cumhuriyet Üniversitesi Adresli:
Evet
Özet
he technique of polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) genotyping was used to characterise morphologically identical isolates of Giardia intestinalis from human stool samples.
Materials and methods:
In this study a total of 17 trophozoite samples, obtained either directly from stool samples or after excystation, or by duodenal aspiration, were used. A set of primers was chosen to amplify the different regions of triose phosphate isomerase (tpi) and a segment of the glutamate dehydrogenase (gdh) genes. A single-stranded conformational polymorphism technique was also used in an attempt to discriminate among some subgroups.Results:
Only primers of the 683-bp segment of the tpi gene from the trophozoite samples were suitable for obtaining a PCR product. In the total of 17 trophozoite DNAs where the tpi gene segment was amplified, 9 belonged to assemblage A (53%) and 4 to assemblage B (23.5%). It was not possible to identify assemblages for the remaining 4 samples (23.5%).Conclusion:
PCR-RFLP tpi gene application was able to discriminate between G. intestinalis assemblage A and B, but not the other subgroups. Since assemblage A is the more prevalent subgroup compared with assemblage B, this subgroup can be said to be responsible for common Giardia infections in Turkey.