Genotyping of Giardia intestinalis isolated from people living in Sivas, Turkey


Değerli S. , Değerli N. , Çeliksöz A. , Özçelik S.

TURKISH JOURNAL OF MEDICAL SCIENCES, vol.42, pp.1268-1272, 2012 (Journal Indexed in SCI) identifier

  • Publication Type: Article / Article
  • Volume: 42
  • Publication Date: 2012
  • Doi Number: 10.3906/sag-1104-32
  • Title of Journal : TURKISH JOURNAL OF MEDICAL SCIENCES
  • Page Numbers: pp.1268-1272

Abstract

he technique of polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) genotyping was used to characterise morphologically identical isolates of Giardia intestinalis from human stool samples.

Materials and methods:

In this study a total of 17 trophozoite samples, obtained either directly from stool samples or after excystation, or by duodenal aspiration, were used. A set of primers was chosen to amplify the different regions of triose phosphate isomerase (tpi) and a segment of the glutamate dehydrogenase (gdh) genes. A single-stranded conformational polymorphism technique was also used in an attempt to discriminate among some subgroups.

Results:

Only primers of the 683-bp segment of the tpi gene from the trophozoite samples were suitable for obtaining a PCR product. In the total of 17 trophozoite DNAs where the tpi gene segment was amplified, 9 belonged to assemblage A (53%) and 4 to assemblage B (23.5%). It was not possible to identify assemblages for the remaining 4 samples (23.5%).

Conclusion:

PCR-RFLP tpi gene application was able to discriminate between G. intestinalis assemblage A and B, but not the other subgroups. Since assemblage A is the more prevalent subgroup compared with assemblage B, this subgroup can be said to be responsible for common Giardia infections in Turkey.