Kaempferol and Isorhamnetin alleviate Lipopolysaccharide-Induced Anxiety and Depression-Like Behavioral in Balb/C Mice: Flavonoids may reverse emotional disorders

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Ekici M., Gungor H., Mert D. G.

JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY, cilt.74, sa.2, ss.5753-5764, 2023 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 74 Sayı: 2
  • Basım Tarihi: 2023
  • Doi Numarası: 10.12681/jhvms.30232
  • Dergi Adı: JOURNAL OF THE HELLENIC VETERINARY MEDICAL SOCIETY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, CAB Abstracts, EMBASE, Veterinary Science Database
  • Sayfa Sayıları: ss.5753-5764
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

The etiology of anxiety and depression is linked to inflammation and oxidative stress. Isorhamnetin

and Kaempferol are strong antioxidants with anti-inflammatory neuroprotective properties. This study, it was aimed to

investigate the effect of Kaempferol and Isorhamnetin in Lipopolysaccharide (LPS)-induced anxiety and depression

model in mice. Thirty Balb/C mice were divided into six groups of five mice each weighing 25-35 g. Kaempferol (50

mg/kg and 100 mg/kg) and Isorhamnetin (30 mg/kg and 60 mg/kg) were given orally to the treatment group, and the

vehicle was given to the control and LPS groups for fourteen days, followed by intraperitoneal (0.83 mg/kg) LPS injection (control group excluding) on the fifteenth day. At 3 hours after the administration of LPS, each group was applied

with the Elevated Plus-Maze (EPM) test, the Light/Dark test, and the Open Field test (OFT). At 24 hours after LPS

administration, the Forced Swimming Test (FST) was applied and at 28 hours, the Tail Suspension Test (TST). After

the behavioral test, the prefrontal cortex and hippocampus tissues of rats were harvested by cervical dislocation under

high-dose anesthesia. From these tissues, malondialdehyde (MDA) levels, total oxidant status (TOS) and total antioxidant status (TAS) levels, tumor necrosis factor alpha (TNF-α), interleukin-1beta (IL-1β), and interleukin-6 (IL-6) levels, and brain-derived neurotrophic factor (BDNF) levels were measured. Kaempferol and Isorhamnetin ameliorated

LPS-induced anxiety evaluated by OFT, Light/Dark test, and EPM, and LPS-induced depression evaluated by FST and

TST. Kaempferol and Isorhamnetin alleviated LPS-induced increased oxidative stress in the prefrontal cortex and hippocampus by decreasing MDA and TOS levels and increasing TAS levels. In addition, it was observed that Kaempferol

and Isorhamnetin regulated the increase in prefrontal and hippocampal inflammation caused by LPS by decreasing

TNF-α, IL-1β, and IL-6 levels. Most importantly, LPS reduced prefrontal and hippocampal BDNF levels, but the treatment groups reversed it. These results show the possible therapeutic potential of Kaempferol and Isorhamnetin, along

with the importance of oxidative stress, inflammation, and BDNF in the etiopathogenesis of anxiety and depression