Mas International European Conference on Mathematics-Engineering-Natural & Medical Sciences-XI, Tokat, Türkiye, 13 - 15 Mart 2020, cilt.1, no.1, ss.68
Aim: Epilepsy is estimated by the World Health Organization to affect 0.8% of the world’s
population. In recent years, an increasing body of evidence has indicated that there is a
complex relationship between epilepsy and the immune system. Adenosine receptors can
modulate neuronal activity and could be a powerful therapeutic target for regulating epileptic
seizures. The aim of this study is to investigate tumor necrosis factor alpha (TNF-α) and
interleukin-1 (IL-1β) levels in the brain of rats in the pentylenetetrazole (PTZ) induced
epileptic models in rats.
Material and Method: In our study, 24 male 220-240 grams Wistar Albino rats were used.
Rats was divided into four groups as control (n=6), Pentylenetetrazole (PTZ 35 mg / kg PTZ
was injected every other day to each group intraperitoneally. According to Racine scale,
animals with 3 seizures at stage 5 and above were considered to be kindled) Adenosine
receptor agonist (A1 agonist) (N6-cyclopentyladenosine 0.3 mg/kg i.p), Adenosine receptor
antagonist (A1 Antagonist) (8-Cyclopentyl-1,3-dimetylxanthine (CPX) 20mg/kg i.p). The
brain tissues of the animals were removed. After the tissues were homogenized, TNF-α and
IL-1β levels were measured in these tissues by using ELISA (enzyme-linked immunosorbent
assay) method. The statistical evaluation was done with one-way ANOVA and multiple
comparisons were determined by the Tukey test.
Results: There was significance increase in TNF-α levels in PTZ group compared to control
(p<0.05). Therefore, the increase in TNF-α in A1 agonist group was more than PTZ group
(p<0.05). On the other hand, there was significance decrease in TNF-α in A1 antagonist
compared to PTZ (p<0.05). Moreover, there was significance increase in IL-1β levels in PTZ
group compared to control (p<0.05). However, A1 antagonist reduced IL-1β compared to
Conclusion: It was found that TNF- α was increased by the A1 agonist and decreased by the
A1 antagonist. IL-1β is reduced by the A1 antagonist.
Keywords: Epilepsy, Pentylenetetrazole, Adenosine Receptor, Tumor Necrosis Factor Alpha,