Composition characterization and biological activity study of Achillea vermicularis extract and extract-loaded nanoparticles.

Doğan M., Koçyiğit Ü. M., Çetin F. N., Yılmaz B. N., Taşkın T.

Biotechnology and applied biochemistry, cilt.70, sa.3, ss.1270-1278, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 70 Sayı: 3
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1002/bab.2437
  • Dergi Adı: Biotechnology and applied biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.1270-1278
  • Anahtar Kelimeler: cytotoxic activity, encapsulation efficiency, enzyme inhibition
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

Antiproliferative activity of Achillea vermicularis extracts was calculated on glial

(C6) and keratinocyte (HaCaT) cell lines using XTT assay. It was observed that

all extracts of A. vermicularis at the determined concentration were not cytotoxic

in HaCaT cell lines. The nanoparticles (NPs) of the extract with the best

cytotoxic activity was prepared, and necessary characterization studies were performed.

Results showed thatNP containing the extract has a lower IC50 value and

more cytotoxic activity in C6 cells compared to the only extract. Furthermore,

the antiepileptic potentials of these substances were explored in this study. The

effect of A. vermicularis extracts on the enzyme activities of carbonic anhydrase

I and II isoenzymes (hCA I and hCA II) was measured using spectrophotometry

to achieve this goal. A. vermicularis extracts demonstrated high inhibitory

activities compared to standard inhibitor (acetazolamide, AAZ), with IC50 values

in the range of 5.04–10.8 μg/ml for hCA I, and 5.40–9.22 μg/ml for hCA

II. High-performance liquid chromatography diode array detector (HPLC-DAD)

was used in this investigation to assess the main chemicals found in the extract

and NPs. The results showed that the ethanol extract (157.636 μg/mg extract) and

NPs (4.631 μg/mg extract) had a significant amount of the 8-hydroxy salvigenin

component.