Screening of the in vitro amoebicidal activities of Pastinaca armenea (Fisch & CAMey.) and Inula oculus-christi (L.) on Acanthamoeba castellanii cysts and trophozoites


DEĞERLİ S. , BERK Ş. , MALATYALI E., TEPE B.

PARASITOLOGY RESEARCH, cilt.110, ss.565-570, 2012 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 110 Konu: 2
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s00436-011-2524-z
  • Dergi Adı: PARASITOLOGY RESEARCH
  • Sayfa Sayıları: ss.565-570

Özet

The aim of this study is to evaluate the in vitro amoebicidal activity of the aqueous extracts of Pastinaca armenea and Inula oculus-christi from Turkey. In the presence of aqueous extracts (ranging from 1.0 to 32.0 mg/ml), numbers of the viable Acanthamoeba castellanii trophozoites and cysts were decreased during the experimental process. Both extracts showed a time- and dose-dependent amoebicidal action on the trophozoites and cysts. Among the extracts tested, I. oculus showed the strongest amoebicidal effect on the trophozoites and cysts. In the presence of 32.00 mg/ml extract solution in the media, no viable trophozoites were determined from the time of 24 h. Moreover, in the presence of 16.00 mg/ml I. oculus-christi extract, no viable trophozoites were detected in 72 h of the experiment. Effectiveness of I. oculus-christi extract was found moderate against the cysts. In the presence of 32.00 mg/ml extract, only 25.3% of the total cysts were killed effectively. In the case of P. armenea, 40.3% and 23.0% of the trophozoites and cysts were killed in the presence of 32.00 mg/ml extract at the end of the experimental process (72 h), respectively.