The fatty acid composition of adipose tissue was measured in 9 Black Pied calves to determine beta-carotene effect. Calves allotted to three groups were fed the diets containing 2 levels of beta-carotene, during the fattening period. All groups were fed ad libitum for six months and slaughtered. Adipose tissue samples were taken from subcutaneous, kidney, pelvic and abdomen and fatty acid composition was determined using gas chromatograph. When beta-carotene level was increased in the feed, the fat of calves became softer and their colour became yellow. The palmitic, oleic percentages changed on feeding beta-carotene and changes in fat location were significantly different (P 0.01). Feeding beta-carotene and fat location affected the total saturated and unsaturated fatty acid percentage significantly (P<0.01). In abdomen and pelvic fat, there was positive correlation between feeding β-carotene and total unsaturated fatty acids and the ratio of unsaturated to saturated fatty acids whereas the correlation between total saturated fatty acids and β-carotene were negative. Addition of β-carotene in feed has increased oleic acid percentages in all fat samples.