Satureja cuneifolia Ten. (wild savoury) belongs to the Lamiaceae family and is used to produce essential oil and aromatic water. This plant is also used as a condiment and herbal tea due to its stimulating, tonic and carminative effects. The in vitro antioxidant, anti-urease, anticholinesterase and cytotoxic activities of the different extracts from the plant’s aerial parts were examined. Besides, the in vivo anti-inflammatory activities of the fraction and direct methanol extracts were determined comparatively. In the current study, fraction methanol extract exhibited the strongest ABTS (52.34 mM trolox/mg extract) radical scavenging and ferric reduction (17.22 mM Fe2+/mg extract) activity. It was also found that the fraction methanol extract had stronger anti-urease (12.52%) and anticholinesterase (69.02%) activity than other extracts. The XTT results showed that fraction methanol extract had the most cytotoxic activity on MCF-7 cell lines (39.92%). According to the results of in vivo anti-inflammatory activity, it was found that both fraction and direct methanol extracts exhibited close and significant anti-inflammatory activity. The fact that methanol extracts have significant biological activity suggests that these may be used as a natural source in the future.