Akademik Veri Yönetim Sistemi
PHARMACEUTICAL CHEMISTRY JOURNAL, cilt.56, ss.54-60, 2022 (SCI-Expanded)
Microorganisms in the biofilm structure cause severe health problems and economic losses. Therefore, it is very important to design and screen anti-biofilm molecules that can solve the related problems. This paper is aimed to demonstrate the antimicrobial and anti-biofilm properties of allyl substituted benzimidazolium-based Ag(I)-containing N-heterocyclic carbene (NHC) complexes. In this work, the antimicrobial and anti-biofilm properties of six allyl-substituted benzimidazolium-based Ag(I)-NHC complexes, the synthesis and characterization of which had been carried out previously, were investigated. The antibacterial properties of Ag(I)-NHC complexes were tested by the broth microdilution method. The biofilm formation was detected by microtiter plate assay. All test compounds (1-6) showed strong and same activity against Escherichia coli ATCC 25922 at concentrations of <= 3.9 mu g/mL as compared to that of the reference anti-biotics (ampicillin). Test compounds exhibited the same or stronger activity than the reference drug against the Acinetobacter baumannii isolate with MedIC values of 15.6 - 31.25 mu g/mL in comparison to the positive control colistin. All compounds inhibited the formation of E. coli biofilms at sub-MIC concentrations by 56 - 90%. Furthermore, it was found that these compounds reduced Klebsiella pneumoniae biofilm formation by 29 - 65%, S. aureus biofilm formation by 36 - 52%, E. faecalis biofilm formation by 38 - 50%, A. baumannii biofilm formation by 51 - 60%, and Candida albicans biofilm formation by 38 - 53%. Regarding the biofilms formed, it was established that the test compounds eradicated the mature of E. coli biofilm by 43 - 63%, K. pneumoniae biofilm by 16 - 40%, S. aureus biofilm by 18 - 39%, E. faecalis biofilm by 15 - 27%, A. baumannii biofilm by 27 - 53%, and C. albicans biofilm by 18 - 41%. Compounds evaluated in our work, which is one of the few studies on benzimidazolium-derived NHCs, have the potential to become new biological agents with strong antimicrobial properties and activity against microbial biofilms. Therefore, further in vivo and in vitro studies should be conducted.