Recent developments in drug technology have led to increasing interest in smart drug systems and systems that provide drug localisation in the area of infection. With the development of various nanoparticles, there have been new studies in this field related to the drug application routes and the effects of nanotechnology products. Drugs modified with nanoparticles have been found to be useful in transporting the drug to the target using systemic, oral, pulmonary, transdermal and other routes, in increasing the bioavailability capacity and protecting stability. The optic, fluorescent and magnetic properties of these nanoparticles are of particular benefit in the early determination of tumour cells and especially in the diagnosis and testing of various pathogenic and genetic diseases. Although studies related to the use of nanoparticles for treatment purposes are still very new, these types of studies have been published in literature in the last few years. The aim of this study was to evaluate the benefit of treatment with antibiotic-coated (enrofloxacin) magnetic nanoparticles added to the outside of a dressing and held on the wound area with the gravitational force of the magnets in an experimental rat model, where an operation wound had been infected with Staphylococcus aureus. Due to the magnetic areas of the magnets placed on the external wound region, the antibiotic-coated nanoparticles were maintained at a high concentration in the wound region, and by showing an effect with a single application without metabolisation in the liver, long-term adverse effects on the liver and kidneys were avoided.