Akademik Veri Yönetim Sistemi
JOURNAL OF APPLIED ORAL SCIENCE, cilt.34, ss.1-9, 2026 (SCI-Expanded)
Exposure to environmental tobacco smoke (ETS) poses significant risks to children’s oral health by inducing oxidative
stress and triggering inflammatory responses. Even in the absence of clinically evident oral inflammation, early biological
changes can be detected in children exposed to ETS. Objectives: This study aimed to evaluate salivary cotinine and
S100A8/A9 levels in children exposed to ETS and to examine the relationship between exposure intensity and inflammatory
biomarker expression. Methodology: This observational case-control study included 150 systemically healthy children
aged 6–12 years. ETS exposure was determined using parent-reported questionnaires. Clinical parameters, including
plaque index, gingival index, and probing pocket depth, were recorded. Unstimulated saliva samples were analyzed for
cotinine, S100A8, and S100A9 using ELISA kits. Group comparisons were conducted using the Mann–Whitney U test, and
correlations were assessed using Spearman’s rank correlation coefficient. Statistical significance was set at p<0.05. Results:
ETS-exposed and non-exposed groups did not differ significantly in age, sex, socioeconomic status, oral hygiene habits,
or clinical periodontal parameters (p>0.05). However, salivary cotinine, S100A8, and S100A9 levels were significantly
higher in ETS-exposed children (p<0.05). Cotinine demonstrated a moderate positive correlation with S100A8, while its
correlation with S100A9 was weak and non-significant. Exposure intensity was positively associated with cotinine and
S100A8 levels, but not with S100A9. Conclusion: ETS exposure in children is associated with early biochemical signs of
inflammation, even in the absence of clinically detectable periodontal changes. Elevated salivary cotinine and S100A8
levels highlight the potential utility of salivary biomarkers for identifying subclinical effects of passive smoking. These
findings underscore the importance of interventions aimed at reducing children’s exposure to ETS to protect long-term
oral health. Clinical trial registration: NCT06791707