Akademik Veri Yönetim Sistemi
SIVAS II. INTERNATIONAL CONFERENCE ON SCIENTIFIC AND INNOVATION RESEARCH, Sivas, Türkiye, 15 - 17 Eylül 2023, ss.319-320
Nowadays, especially viral infectious diseases are the most common clinical problems in captive psittacine birds due to their mortality, difficulties in their treatment and control. One of the most common of these viral diseases in budgerigars is Gammapolyomavirus avis (Budgerigar fledgling disease virus/BFDV). BFDV infection results in a high mortality rate of up to 100% in nestling birds, while adults only present with symptoms such as mild feather dystrophies (French moult). Gammapolyomavirus avis; non-enveloped, icosahedral DNA viruses with a diameter of 40-45 nm, belonging to the Gammapolyomavirus genus of the Polyomaviridae family. The double-stranded and circular genome consists of approximately 5.000 nucleotides. This study was carried out for the molecular detection and genomic characterization of BFDV detected in budgerigars brought to our Virology Department with the complaint of death in baby budgerigars in different time periods in 2022. For this purpose, flocks of 10-20 couples birds belonging to three different amateur breeders with BFDV infection detected in Sivas province constituted our study material. Cloacal swab samples were taken from five couples of adult budgerigars (30 in total) and tissue samples from 10 nestling birds from each of the 3 flocks with offspring. Swab samples were diluted 1/10 with sterile PBS solution and centrifuged. Tissue samples, on the other hand, were crashed and homogenized in sterile mortar, diluted 1/10 and subjected to centrifugation. Viral DNAs were purified from the obtained supernatants with a commercial DNA extraction kit. The obtained DNA samples were amplified with primer sets designed for this study targeting the VP1 and T antigen genes of BFDV. PCR products were electrophoresed on a 1.5% agarose gel stained with ethidium bromide. Partial sequence analysis of the obtained 456 bp VP1 and 404 bp T antigen gene regions was performed. As a result, in this study, it has been shown by molecular methods that the cause of these losses is BFDV in the flocks of amateur breeders who have lost nestling budgerigars. In addition, partial sequence analyzes of VP1 and T antigen genes of BFDV were performed and molecular characterization of BFDV isolates observed in Türkiye was performed.