The arbuscular mycorrhizal fungi (AMF) are obligated to the host plant, thus sufficient and healthy mycorrhizal inoculum production through the host plant is important. Growth mediums are also crucial for mycorrhizal spore propagation. The aim of this research is to determine the suitable mycorrhizal spore doses and root infection percentage for optimum plant growth at various host plants at different harvest times, along with mycorrhizal species and application doses. Three different plant family groups such as graminae (maize, sorghum), alliaceae (leek, onion), leguminosea (clover and alfalfa) were used as host plants and Funnelifortnis mosseae and Claroideoglomus etunicatum were used as mycorrhiza species with different spore doses (Control, 500-1000 and 2000 spore pots(-1)). Plants were harvested the 3rd, 4th, 5th and 8th weeks following sowing for determining root infection percentage (%), and spore production.