Akademik Veri Yönetim Sistemi
TURKISH JOURNAL OF AGRICULTURE: FOOD SCIENCE AND TECHNOLOGY, cilt.10, sa.10, ss.2056-2060, 2022 (Hakemli Dergi)
In this study; it was aimed to investigate the anticancer and cytotoxic activities on DU-145 prostate
cancer and MCF-7 breast cancer cell lines and WI-38 human fibroblast cell line of essential oil
(Nioli) obtained from Melaleuca viridiflora Sol. ex Gaertn. plant and its antimicrobial effects on
various bacteria and yeast cells. Content analyzes of Nioli essential oil were made by Gas
Chromatography-Mass Spectrometry (GK-KS). The antimicrobial effects of the essential oil were
determined using Disk Diffusion and Minimum Inhibition Concentration (MIC) tests. Its cytotoxic
effects were determined by the XTT (2,3-bis-(2-methoxy-4-) nitro-5)-sulfophenyl)-2H-tetrazolium5-carboxanilide) test; were investigated at different concentrations in breast cancer (MCF-7),
prostate cancer (DU-145) and healthy human fibroblast (WI-38) cell lines. In the disc diffusion
method of essential oil; It was determined that it formed an inhibition zone against Klebsiella
pneumoniae bacteria at 200, 100, 50, 25 and 12.5 µg/mL concentrations, and against Candida
albicans yeast at 200 and 100 µg/mL concentrations. Nioli essential oil showed the highest
antibacterial effect against Bacillus cereus. While it was moderately effective against Pseudomonas
aeruginosa and Candida albicans, it reached MIC values effective against other bacteria. It was
observed that the anticancer activity of Nioli essential oil was more effective in DU-145 prostate
cancer cells compared to MCF-7 breast cancer cells, and it had no toxicity in WI-38 healthy human
fibroblast cells.