DNA interaction, antitumor and antimicrobial activities of three-dimensional chitosan ring produced from the body segments of a diplopod

Kaya M., Akyuz B., Bulut E., Sargin I., Tan G., Erdonmez D., ...Daha Fazla

CARBOHYDRATE POLYMERS, cilt.146, ss.80-89, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 146
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1016/j.carbpol.2016.03.033
  • Dergi Adı: CARBOHYDRATE POLYMERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.80-89
  • Anahtar Kelimeler: Millipede, Chitin, Biopolymer, Cytotoxicity, HeLa cell line, Antibacterial, MOLECULAR-WEIGHT, PORIFERAN CHITIN, GENE DELIVERY, ALPHA-CHITIN, DERIVATIVES, EXTRACTION, SCAFFOLDS, IDENTIFICATION, PROLIFERATION, DEACETYLATION
  • Sivas Cumhuriyet Üniversitesi Adresli: Hayır

Özet

Commercially available chitins and the chitin isolated from mushrooms, insect cuticles, shells of shrimp, crab and crayfish reported in the literature are in forms of powder, flake or granule. Three-dimensional chitins have been only known from the sponges but still three-dimensional chitosan has not been reported yet. In this study, we produced three-dimensional chitin and chitosan rings from the body segments of a diplopod species (Julus terrestris). Obtained chitin and chitosan rings were characterized (by FT-IR, SEM, TGA, XRD, dilute solution viscometry and EA) and compared with commercial chitin and chitosan. The interactions with plasmid DNA was studied at varying concentrations of chitosan (0.04, 0.4 and 4 mg/mL). Antitumor activity tests were conducted (L929 and HeLa), low cytotoxicity and high antiproliferative activity was observed. Antimicrobial activities of J. terrestris chitosan were investigated on twelve microorganisms and maximum inhibition (15.6 +/- 1.154 mm) was recorded for common human pathogen Staphylococcus aureus. (C) 2016 Elsevier Ltd. All rights reserved.