In vitro cytotoxic activities of Plantago major L. plants from Sivas (Yıldızeli) province

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Daştan T. , Durna Daştan S. , Kaya Ş., Çevik Ö.

Webinar on Life Sciences (International congress on life sciences), New York, Amerika Birleşik Devletleri, 26 - 28 Ocak 2021, ss.53-59

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Basıldığı Şehir: New York
  • Basıldığı Ülke: Amerika Birleşik Devletleri
  • Sayfa Sayıları: ss.53-59


Plantago major L. is perennial plant that belongs to the Plantaginaceae family. More recent ethnopharmacological studies show that Plantago plants are used in the treatment of a number of diseases by using as an anesthetic, antiviral, anti-inflammatory, astringent, anti-helmintic, analgesic, antihistaminic, anti rheumatic, antitumor, anti-ulcer, diuretic, expectorant and hypotensive in traditional medicine in almost all over the World. In this study, Plantago major plant materials were collected from Yıldızeli, Sivas province in spring-summer season of 2020 year. Identification of the plants were performed by Doç. Dr. Mustafa Sevindik. Water and ethanol were used as extraction solvents and extraction yield was 6.96 and 5.81 %w/w respectivelly. Human gastric cancer cell line (MKN-45), Human prostate cancer cell line, (PC3), Mouse fibroblast cell line (L929), Human cervical cancer cell line (HeLa),  Human gliablastoma cell line (U87) and human colon adenocarcinoma (HT29) cell lines were used for cytotoxic activities by using MTT cell proliferation kit. As a result of MTT experiments, IC50 values ​​were calculated using Graphpad program. SPSS 22.0 (IBM Corparation, Armonk, New York, United States) program was used for statistical data analysis. Both plant extracts inhibited HeLa and U87 cell lines in medium level and there was a low inhibition on MKN-45, PC3, L929, HT29 cell lines’ proliferation.


Key words: cytotoxic activity, Plantago major, extraction, ethnopharmacology.