In this study the aim is to determine the blood serum levels of biological markers as procalcitonin, neopterin, TNF-alpha, MDA, PGE2, IL8, IFN-gamma which are considered as highly beneficial on diagnosing the infections in the veterinary medicine and evaluating the prognosis in the healthy cattle at different ages and in different gender. The materials of this study are 48 (25 female and 23 male) cattle and calf bred (neonatal <1 month, young 12-24 month and mature >24 month) in operations in Sivas region and which are determined to be healthy via the biochemical and hematological findings. Serum procalcitonin level was found lower in neonatal group than the young and adult group (P<0.05). It was realized that neopterin level is reasonably higher in neonatal group than both young and adult group (P<0.05). MDA level in the young and adult group was measured as higher than of the neonatal group (P<0.05). There was found between the adult group and neonatal group a statistically reasonable difference for the serum PGE2 levels (P<0.05). In IL-8 level, there was found a statistically important difference only between young group and neonatal group (P<0.05). There was found no statistic difference among the levels of procalcitonin, neopterin, TNF-alpha, IFN-gamma, MDA, PGE2, IL-8 between the sexes. As a result, it is concluded that determining the levels of markers used in defining the prognosis of the infection in healthy cattle at different ages would be a base data for further studies.