A morphological, anatomical, palynological, and karyological of Silene commelinifolia var. ruscifolia, an endemic taxon for Türkiye, was examined in the present study. The anatomical approach described the root from outside to inside, by the periderm, cortex, endodermis, perisycle tissues, and vascular system. The stem is formed by cuticula layer, epidermis tissue, cortex tissue, sclerenchymatical rings and vascular system. The leaves, sepals and petals are formed by cuticula layer, epidermis tissue which is at the bottom, and the upper surface of the leaf and mesophyll tissue. The length of the root 30- 130 mm and the diameter of the root 6-22 mm, type of root taproot, stem erect, and cylindric, the length of the stem is 46-156 mm and the diameter of stem 20-35 mm, intensive glandular puberulous, cauline leaves ovate-cordate, 6-34 x 1-25 mm, intensive glandular puberulous, basal leaves linear- oblanceolate, 6-69 x 1-9 mm, intensive glandular puberulous, the flower monocline, diclamideic, fruit 9-18 mm, denticid capsula, seeds reniform, brown, at the size of 1.62-2.17 x 1.25-1.67 x 0.75-1.125 mm. Pollen periporate, prolate-spherical (A/B=1.03), long axis of pollen (A) 31.18 μm, short axis of pollen (B) 30.14 μm, structure tektat, ornamentation microechinat-perphorate, number of pores 24- 34, the length of pore (Plg) 4.95 μm, the width of pore (Plt) 4.18 μm, distance between pores 5.34 μm, the width of ecsin 2.23 μm on the average. In our study, it was determined that the chromosome number of the species was 2n=24.