Akademik Veri Yönetim Sistemi
Journal of Food Composition and Analysis, cilt.132, 2024 (SCI-Expanded)
A sensitive and simple ultrasonic assisted microextraction procedure based on the use of green deep eutectic solvents (DESs) was designed for the extraction of zearalenone (ZEN) residues in grains, foods and milks. Optimization of key parameters and experimental modelling were achieved by Box-Behnken design (BBD) based on surface response methodology. DES-UA-ME procedure was based on the extraction of ZEN molecules into DES prepared from a mixture of phenyl acetic acid and trimethyl glycine in a 2:1 molar ratio at pH 3.8 under ultrasonication for 11 min at 33 °C. After the phase separation step, the DES-rich phase containing ZEN was placed in an UV-Vis spectrophotometer for quantitative analysis of ZEN at 260 nm. The analytical merits of the DES-UA-ME procedure were investigated using optimum conditions for both standard calibration solutions (SCS) and matrix-matched calibration solutions (MMCS). Linearity for the SCS and MMCS was 3–800 ng mL−1 (r2:0.995) and 5–450 ng mL−1 (r2:0.992), respectively. The limit of detection (LOD) and limit of quantification (LOQ) for SCS were 0.9 ng mL−1 and 3 ng mL−1, respectively, while the LOD and LOQ for MMCs were 1.6 ng mL−1 and 5 ng mL−1, respectively. Enrichment factors for SCS and MMCS was 157 and 139 for, respectively. Validation of the DES-UA-ME procedure was tested by analysis of two certified reference materials containing ZEN. There was no significant difference between the measured amounts and the reference amounts. In the final stage, the DES-UA-ME procedure was applied to selected samples separately for both SCS and MMCS.