Akademik Veri Yönetim Sistemi
Gene Reports, cilt.40, 2025 (ESCI)
Insufficient placental blood perfusion is recognized as a contributing factor to preeclampsia (PE), resulting in persistent placental hypoxia. Under hypoxic conditions, certain microRNAs (miRNAs) exhibit varying expression levels. The literature suggests that miR-210 is integral to hypoxia response pathways, including angiogenesis, mitochondrial respiration, cell survival, and DNA repair. Conversely, miR-383 is believed to modulate the fundamental effects of hypoxia-inducible factor 1-alpha (HIF-1α) by targeting genes such as vascular endothelial growth factor and lactate dehydrogenase-A, which are regulated by HIF-1α in endothelial cells. This study aimed to evaluate miR-210 and miR-383 expression levels, anticipated to change under hypoxic conditions, using quantitative polymerase chain reaction (qPCR) in maternal blood (MB), placental tissue (PT), and umbilical cord blood (CB) samples from women with preeclampsia (PE) (N = 22) and control (N = 15). The study findings indicate that the expression of miR-210 increased, while the expression of miR-383 decreased in all samples from the PE group compared to the control group. In PE subgroups, miR-210 levels increased in MB by 3.78-fold and 2.73-fold, in PT by 3.67-fold and 2.49-fold, and in CB by 5.48-fold and 5.04-fold in the severe and mild PE groups, respectively (p < 0.01). In contrast, a significant decrease in miR-383 expression was observed only in PT, with a 2.22-fold reduction (p = 0.01) in the severe PE group and a 7.69-fold reduction (p = 0.002) in the mild PE group. Evaluations comparing the severe and mild PE groups revealed significant differences in the expression of miR-210 in MB and PT, as well as miR-383 in PT (p = 0.01, p < 0.001, and p = 0.001, respectively). According to the ANOVA test, miR-210 showed significant differences across all samples, while miR-383 showed significance only in PT (p < 0.001). These findings suggest that miR-210 plays a key role in PE pathophysiology under hypoxic conditions, while miR-383 reduction, particularly in mild PE, may contribute to the condition's progression.