This study was designed to examine the in vitro antimicrobial and antioxidant activities of the essential oil of T. boveii and T. hyemalis. According to the results of GC-EIMS analysis, essential oils were found rich in phenols and hydrocarbons. p-cymene, thymol and carvacrol were mainly found as the major compounds for the essential oils. Both plant species showed remarkable antioxidant activity in all test systems except chelating effect. In the case of antimicrobial activity, the oils showed remarkable growth inhibition against the tested microorganism except K. pneumoniae, P. aeruginosa, L. monocytogenes, P. fluorescens.