Using Safranin T as a Charge Transfer-Sensitive Ion-Pairing Reagent in Ultrasound-Assisted Cloud Point Extraction: Determination of Bisphenol A in Selected Beverages


Temel N. , GÜRKAN R.

JOURNAL OF AOAC INTERNATIONAL, cilt.101, ss.277-287, 2018 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 101 Konu: 1
  • Basım Tarihi: 2018
  • Doi Numarası: 10.5740/jaoacint.17-0106
  • Dergi Adı: JOURNAL OF AOAC INTERNATIONAL
  • Sayfa Sayıları: ss.277-287

Özet

In this study, a new ultrasonic-assisted cloud point extraction method coupled with spectrophotometry was developed to extract from and determine bisphenol A (BPA) in a sample matrix. The method is based on charge transfer-sensitive ion-pairing complex formation between BPA and Safranin T in the presence of nitrate in a micellar interface at pH 5.0. The variables affecting extraction efficiency were optimized. A good linear relationship with a significant sensitivity difference was obtained in the ranges of 2-40 and 2-120 mu g/L with LODs of 0.54 and 0.38 mu g/L after preconcentration with two different extractants, respectively. From a preconcentration of a 15 mL sample, a preconcentration factor of 60 was obtained. Due to the lack of a certified material compatible with the sample matrix, the method was validated by conducting intraday and interday accuracy and precision studies based on two spiked QC samples (with a recovery rate greater than 94% and an RSD ranging from 3.8 to 5.9%). Moreover, the beverage samples were analyzed by the standard addition method to control for possible matrix effects. BPA was detected in the range of 1.8-7.2 mu g/L in beverages with Triton X-114, whereas the levels changed to a range of 1.8-7.3 mu g/L with Triton X-45. In such a way not to create a threat, these levels were considerably lower than the specific migration limit set by the European Union.